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Quiz time/ 03

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Quiz time/ 03

Post by Admin on Thu Oct 15, 2015 3:37 pm

MCQ'S - gel electrophoresis

1. In an SDS-PAGE

A. proteins are denatured by the SDS
B. proteins have the same charge-to-mass ratio
C. smaller proteins migrate more rapidly through the gel
D. all of the above

2. Electrophoresis is used to

A Separate DNA fragments.
B Identify DNA fragments.
C Purify DNA fragments.
D Both A and B
E Both A and C

3. The rate at which DNA migrates through the gel is determined by:

A Molecular size of the DNA and the agarose gel concentration.
B Conformation of DNA and the applied voltage.
C The length of the agarose gel and the negativity of the DNA.
D Both A and B
E Both A and

4. Why do scientists load DNA of known sizes into the agarose gel?
A It makes it easier to determine sizes of unknowns using comparison techniques.
B To fill in all the slots on the gel so you can run it.
C To practice loading the DNA before you get to the important DNA.
D So you know how long the gel needs to run.

5. How can one tell if their gel electrophoresis is running properly?
A It bubbles.
B You can see the methyl blue move from the well into the gel.
C The DNA runs to red
D Both B and C
E All three choices A, B and C
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Re: Quiz time/ 03

Post by Ravindee on Sun Nov 22, 2015 9:18 am

1) D. all of the above

2) E Both A and C

3) D. Both A and B

4) A. It makes it easier to determine sizes of unknowns using comparison techniques.

5) E. All three choices A, B and C
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