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Sterilization - Fact#03

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Sterilization - Fact#03

Post by Admin on Fri Sep 25, 2015 10:22 pm

WET HEAT (Autoclaving)

The method of choice for sterilization in most labs is autoclaving; using pressurized steam to heat the material to be sterilized. This is a very effective method that kills all microbes, spores and viruses, although for some specific bugs, especially high temperatures or incubation times are required.

Autoclaving kills microbes by hydrolysis and coagulation of cellular proteins, which is efficiently achieved by intense heat in the presence of water.

DRY HEAT (Flaming, baking)

Dry heat utilizes hot air that is either free from water vapour, or has very little of it in the process of sterilization. Dry heating has one crucial difference from autoclaving. There’s no water, so protein hydrolysis can’t take place.

Instead, dry heat tends to kill microbes by oxidation of cellular components. This requires more energy than protein hydrolysis so higher temperatures are required for efficient sterilization by dry heat.
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